Job Description

A small stealth biotech company in downtown Seattle is seeking a highly motivated Scientist or Senior Scientist to join our dynamic team. The ideal candidate will be responsible for protein expression, purification, characterization, and analysis, with a focus on protein-protein interactions, biophysical techniques (e.g., BLI, SPR), and coordination of internal and external (CRO) work. Prior experience with antibodies and bispecifics is highly desirable.

Key Responsibilities

Protein Expression and Purification:

• Design constructs for recombinant protein expression in bacterial, insect, or mammalian systems.
• Optimize protocols for high-yield and high-purity protein production.
• Perform purification using techniques such as affinity chromatography, ion exchange (IEX), hydrophobic interaction chromatography (HIC), and size exclusion chromatography (SEC).

Protein Characterization and Analysis:

• Conduct biophysical studies using BLI (Bio-Layer Interferometry) and SPR (Surface Plasmon Resonance) to study protein-protein interactions.
• Perform quality control assays including SDS-PAGE, Western blotting, UV-Vis spectroscopy, and dynamic light scattering.
• Analyze protein stability and aggregation using advanced techniques like SEC-MALS or differential scanning fluorimetry.

Antibody and Bispecific Development:

• Express and purify monoclonal antibodies and bispecific molecules.
• Characterize binding affinities and functional properties of antibody-based therapeutics.

Coordination with CROs:

• Manage external collaborations with Contract Research Organizations (CROs) for protein production and characterization.
• Troubleshoot challenging proteins and ensure timely delivery of results.

Cross-functional Collaboration:

• Work closely with interdisciplinary teams including structural biologists, assay development scientists, and therapeutic project leads.
• Communicate progress, challenges, and results effectively to internal stakeholder.

Qualifications

Education:

• Ph.D. in Biochemistry, Structural Biology, Biophysics, or related field with at least 3–5 years of relevant industry experience.
• Candidates with an M.S. or B.S. degree and significant hands-on experience will also be considered.

Technical Expertise:

• Proven track record in recombinant protein expression, purification, and characterization.
• Hands-on experience with biophysical techniques such as BLI or SPR is required.
• Familiarity with antibody engineering or bispecific design is strongly preferred.

Skills:

• Strong problem-solving skills for troubleshooting complex protein-related challenges.
• Excellent organizational skills with attention to detail for documentation in electronic lab notebooks (ELN).
• Ability to work independently as well as collaboratively in a fast-paced environment.

Additional Desired Skills:

• Experience in managing external collaborations (e.g., CROs).
• Knowledge of structural biology methodologies such as X-ray crystallography or cryo-EM is a plus.
• Familiarity with automation tools for protein purification is advantageous.

Benefits:

The company offers a competitive compensation package including salary, performance bonuses, equity options, comprehensive health benefits, and opportunities for professional growth in a collaborative environment.

This role provides an exciting opportunity to contribute to the development of innovative therapeutic proteins in a rapidly growing biotech company.

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